The research of new botanicals is an important aspect of the modern research focused on prevention and cure of cancer. This project evaluated the in vitro geno-protective capacity (SOS-Chromotest) and anti-proliferative activity (MCF7, A549, MDA-MB-231, LoVo, HepG2, K562, Jurkat and IB3-1 cell lines) of two traditional preparations, decoction and mother tincture, of two ayurvedic crude drugs: Hemidesmus indicus roots and Azadirachta indica leaves. Our previous results demonstrated an interesting antileukemic effect1,2 of H. indicus decoction. In present study both H. indicus preparations possess anti-proliferative activity against all the cell lines considered. Among phytochemical markers, the most active, 2-hydroxy-4-methoxybenzaldehyde, showed an IC50 of 12.09±2.78 μg/ml against K562 and an IC50 of 13.15±5.57 μg/ml against Jurkat cells. The only relevant data for A. indica were against: MDA-MB-231 cell (IC50= 381.57±43.62 μg/ml) for decoction and K562 (IC50= 276.05±26.60 μg/ml) and Jurkat cells (IC50= 207.18±29.97 μg/ml) for mother tincture. Further analyses of anti-proliferative bioactivity for A. indica are still in progress. In conclusion: H. indicus preparations were more effective than those of A. indica; mother tincture of both crude drugs showed a general wider anti-proliferative activity than decoction, in particular for H. indicus, it evidenced promising data against Jurkat (63.79±7.97 μg/ml), Hep-G2 (34.50±0.14) and LoVo (29.84±0.24); H. indicus mother tincture and decoction were more effective than 2-hydroxy-4-methoxybenzaldehyde against Hep-G2 cell, pointing out possible synergistic (agonistic) activity of minor compounds.
In vitro evaluation of the anti-proliferative and geno-protective activity of traditional preparations of ayurvedic crude drugs
TACCHINI, Massimo;BROGNARA, Eleonora;Roberto Gambari;GUERRINI, Alessandra;SACCHETTI, Gianni
2013
Abstract
The research of new botanicals is an important aspect of the modern research focused on prevention and cure of cancer. This project evaluated the in vitro geno-protective capacity (SOS-Chromotest) and anti-proliferative activity (MCF7, A549, MDA-MB-231, LoVo, HepG2, K562, Jurkat and IB3-1 cell lines) of two traditional preparations, decoction and mother tincture, of two ayurvedic crude drugs: Hemidesmus indicus roots and Azadirachta indica leaves. Our previous results demonstrated an interesting antileukemic effect1,2 of H. indicus decoction. In present study both H. indicus preparations possess anti-proliferative activity against all the cell lines considered. Among phytochemical markers, the most active, 2-hydroxy-4-methoxybenzaldehyde, showed an IC50 of 12.09±2.78 μg/ml against K562 and an IC50 of 13.15±5.57 μg/ml against Jurkat cells. The only relevant data for A. indica were against: MDA-MB-231 cell (IC50= 381.57±43.62 μg/ml) for decoction and K562 (IC50= 276.05±26.60 μg/ml) and Jurkat cells (IC50= 207.18±29.97 μg/ml) for mother tincture. Further analyses of anti-proliferative bioactivity for A. indica are still in progress. In conclusion: H. indicus preparations were more effective than those of A. indica; mother tincture of both crude drugs showed a general wider anti-proliferative activity than decoction, in particular for H. indicus, it evidenced promising data against Jurkat (63.79±7.97 μg/ml), Hep-G2 (34.50±0.14) and LoVo (29.84±0.24); H. indicus mother tincture and decoction were more effective than 2-hydroxy-4-methoxybenzaldehyde against Hep-G2 cell, pointing out possible synergistic (agonistic) activity of minor compounds.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
