A method for microencapsulation of isolated neonatal porcine Sertoli cells is described. Using a conventional alginate-poli-L-ornithine encapsulation procedure, which has been used in our laboratory for almost two decades to envelop pancreatic islets, we observed significant loss of Sertoli cell viability, possibly due to excessive Ca2+ ion exposure. Replacing calcium with barium, or shortening the incubation period in the presence of Ca ions, we obtained barium or calcium alginate gel microbeads that did not alter morphology and viability of the encapsulated Sertoli cells. The procedure might permit access to a novel approach to immunologically alter cell graft acceptance. © Mary Ann Liebert, Inc.

Encapsulation, in vitro characterization, and in vivo biocompatibility of sertoli cells in alginate-based microcapsules

NASTRUZZI, Claudio;
2007

Abstract

A method for microencapsulation of isolated neonatal porcine Sertoli cells is described. Using a conventional alginate-poli-L-ornithine encapsulation procedure, which has been used in our laboratory for almost two decades to envelop pancreatic islets, we observed significant loss of Sertoli cell viability, possibly due to excessive Ca2+ ion exposure. Replacing calcium with barium, or shortening the incubation period in the presence of Ca ions, we obtained barium or calcium alginate gel microbeads that did not alter morphology and viability of the encapsulated Sertoli cells. The procedure might permit access to a novel approach to immunologically alter cell graft acceptance. © Mary Ann Liebert, Inc.
2007
G., Luca; M., Calvitti; Nastruzzi, Claudio; L., Bilancetti; E., Becchetti; G., Angeletti; F., Mancuso; R., Calafiore
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1683328
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