Clustering of undermethylated CCGG and GCGC sequences in the 5' region of the Ha-ras-1 oncogene of human leukemic K562 cells

Barbieri, R.; Piva, Maria Roberta; Buzzoni, D.; Volinia, Stefano; Gambari, Roberto

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The methylation state of the CCGG and GCGC sites of the Ha-ras-1 oncogene was analysed in the human leukemic K562 cell line, which was found to actively transcribe this gene. The results obtained demonstrate that the Ha-ras-1 oncogene is extensively methylated in both exonic, intronic, VTR and 3′ untranslated portions, while undermethylations are present in a CG-rich island localized upstream of exon 1, near putative transcription initiation signals. Treatment of K562 cells with 5-azacytidine induces undermethylation of the Ha-ras-1 oncogene without major differences in the accumulation of Ha-ras-1 mRNA trascripts.

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Titolo:	Clustering of undermethylated CCGG and GCGC sequences in the 5' region of the Ha-ras-1 oncogene of human leukemic K562 cells
Autori:	BARBIERI R. PIVA, Maria Roberta BUZZONI D. VOLINIA, Stefano GAMBARI, Roberto mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	1987
Rivista:	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Abstract:	The methylation state of the CCGG and GCGC sites of the Ha-ras-1 oncogene was analysed in the human leukemic K562 cell line, which was found to actively transcribe this gene. The results obtained demonstrate that the Ha-ras-1 oncogene is extensively methylated in both exonic, intronic, VTR and 3′ untranslated portions, while undermethylations are present in a CG-rich island localized upstream of exon 1, near putative transcription initiation signals. Treatment of K562 cells with 5-azacytidine induces undermethylation of the Ha-ras-1 oncogene without major differences in the accumulation of Ha-ras-1 mRNA trascripts.
Handle:	http://hdl.handle.net/11392/1682550
Appare nelle tipologie:	03.1 Articolo su rivista

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