The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon (IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-B1/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-B1/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-B1/B2 is not modulated by IFN-γ treatment
Binding of nuclear factors to the IFN-γ consensus sequence of the human HLA-DRα gene: effects of IFN-γ on tumor cell lines
GAMBARI, Roberto;NASTRUZZI, Claudio;FERIOTTO, Giordana;PIVA, Maria Roberta;
1990
Abstract
The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DRα gene was studied. As the HLA-DRα gene is inducible by interferon (IFN)-γ, nuclear extracts were also purified from IFN-γ treated cells. Our data indicate that a) nuclear binding proteins (named IFN-γ-B3 and Z-B1/B2) are detectable, specific for the IFN-γ and Z boxes of the human HLA-DRα gene; b) both IFN-γ-B3 and Z-B1/B2 are present in HLA-D negative cell lines and c) the content of IFN-γ-B3 and Z-B1/B2 is not modulated by IFN-γ treatmentFile in questo prodotto:
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