Rice plants and cell cultures were relatively highly resistant to the growth-inhibitory prolineanalogue, azetidin-2-carboxylicacid (A2CA). The biochemical steps possibly involved in the resistance to the analogue in cell cultures of rice have been compared with those of carrot, a relatively A2CA-sensitive species. No significant differences were found in the extent of inactivation, uptake, transfer to tRNA and incorporation of A2CA into protein. Also the intracellular content of free proline was found to be similar in the two species. A significant difference, however, was observed in the extent of conversion of glutamate into proline by whole cells in the presence of A2CA. Under these conditions inhibition of proline biosynthesis occurred only in carrot suggesting that feedback control of the proline pathway by A2CA does not operate in rice.
Biochemical characterization of the natural resistance of rice to the proline analogue azetidin–2–carboxylic acid.
FORLANI, Giuseppe;
1986
Abstract
Rice plants and cell cultures were relatively highly resistant to the growth-inhibitory prolineanalogue, azetidin-2-carboxylicacid (A2CA). The biochemical steps possibly involved in the resistance to the analogue in cell cultures of rice have been compared with those of carrot, a relatively A2CA-sensitive species. No significant differences were found in the extent of inactivation, uptake, transfer to tRNA and incorporation of A2CA into protein. Also the intracellular content of free proline was found to be similar in the two species. A significant difference, however, was observed in the extent of conversion of glutamate into proline by whole cells in the presence of A2CA. Under these conditions inhibition of proline biosynthesis occurred only in carrot suggesting that feedback control of the proline pathway by A2CA does not operate in rice.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
