In the human embryonic development, the umbilical cord comes from trofoblast, the embryo part that will not originate differentiated tissues but is committed to organize the trophic system for the developing embryo. The easy collecting procedure of the cord stroma and the plasticity of the cells isolated make it a suitable source for the collection of human mesenchymal cells. At the present moment, the bone needing for clinical therapies is increasing and identification of an alternative human cellular resource, easy to collect and able to produce a well-differentiated extracellular bone matrix, acts in this way. In this paper we demonstrate that the cord stroma mesenchymal progenitors are easy to commit into osteogenic lineages after exposition to conditionated medium of Runx2+/OC+positive cells, obtained from differentiation of human dental pulp stem cell, cells that are bone committed cells and can be used to studythe bone regeneration mechanisms. The chance to obtain the osteogenic differentiation and the production of a well-differentiated bone matrix just culturing the cells with the conditionated medium of Runx2+/OC+ cells is very important in the perspective of clinical application of these MSC

HUMAN CORD STROMA CELLS AFTER CO-CULTURE WITH HUMAN DENTAL PULP CELLS UNDERGO TO OSTEOGENIC DIFFERENTIATION THROUGH A BMP 2-MEDIATED PATHWAY

CARINCI, Francesco
2011

Abstract

In the human embryonic development, the umbilical cord comes from trofoblast, the embryo part that will not originate differentiated tissues but is committed to organize the trophic system for the developing embryo. The easy collecting procedure of the cord stroma and the plasticity of the cells isolated make it a suitable source for the collection of human mesenchymal cells. At the present moment, the bone needing for clinical therapies is increasing and identification of an alternative human cellular resource, easy to collect and able to produce a well-differentiated extracellular bone matrix, acts in this way. In this paper we demonstrate that the cord stroma mesenchymal progenitors are easy to commit into osteogenic lineages after exposition to conditionated medium of Runx2+/OC+positive cells, obtained from differentiation of human dental pulp stem cell, cells that are bone committed cells and can be used to studythe bone regeneration mechanisms. The chance to obtain the osteogenic differentiation and the production of a well-differentiated bone matrix just culturing the cells with the conditionated medium of Runx2+/OC+ cells is very important in the perspective of clinical application of these MSC
2011
A., Graziano; R., D’Aquino; L., Visai; V., Maliardi; L., Benedetti; M. G., CUSELLA DE ANGELIS; M., Aimetti; Carinci, Francesco
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1506315
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