Several evidences support the involvement of the immune system during ovulation, commonly considered as an inflammatory reaction. During this process the oocyte acquires quality and competence that will support the early stages of embryo development. Our knowledge on human oocyte protein content is still limited because of the difficulties in analyzing it. The in vitro oocyte maturation procedure, where the oocyte is in vitro maturated, has allowed the analysis of cumulus-oocyte complex (COC) secretome without the influence of the in vivo maternal microenvironment. We have evaluated 112 in vitro maturated human COCs culture supernatants for the presence of 20 different soluble anti and pro-inflammatory molecules (soluble HLA-G, IL1-ra, IL-9, IL-15, eotaxin, IP10, PDGF, RANTES, VEGF, IL1b, IL- 6, IL-7, IL-8, IL-10, IL-13, G-CSF, MCP1, MIP1, ICAM1) by means of Bio-plex system (Bio-Rad). We have observed a significant correlation between oocyte maturation and the secretion of two immunosoppressive molecules: soluble HLA-G (sHLAG) and sICAM-1. sHLA-G has been observed in 14/73 (19%) mature COCs (300 - 800 pg/mL/24 hours) while no sHLA-G have been revealed in immature COCs (n = 39). sICAM-1 has higher levels in immature oocytes (6711.5 +/− 1502.4 pg/mL/24 hours) while it decreases during maturation (2987 +/− 103.7 pg/mL/24 hours). Interestingly these two molecules are inversely correlated (p = 0.0369; Spearman Correlation test) proposing a temporal control of molecule secretion to regulate the inflammatory microenvironment during oocyte maturation. These results support the concept that oocyte are protected from immunological events by the expression of sICAM and sHLA-G molecules which could act as immunosuppressive against the adhesion of cytotoxic lymphocytes and cell-mediated immunity respectively.

Implication of immunosoppressive molecules during human oocyte maturation.

RIZZO, Roberta;STIGNANI, Marina;BORGATTI, Monica;GRAPPA, Teresa;GAMBARI, Roberto;BARICORDI, Olavio
2010

Abstract

Several evidences support the involvement of the immune system during ovulation, commonly considered as an inflammatory reaction. During this process the oocyte acquires quality and competence that will support the early stages of embryo development. Our knowledge on human oocyte protein content is still limited because of the difficulties in analyzing it. The in vitro oocyte maturation procedure, where the oocyte is in vitro maturated, has allowed the analysis of cumulus-oocyte complex (COC) secretome without the influence of the in vivo maternal microenvironment. We have evaluated 112 in vitro maturated human COCs culture supernatants for the presence of 20 different soluble anti and pro-inflammatory molecules (soluble HLA-G, IL1-ra, IL-9, IL-15, eotaxin, IP10, PDGF, RANTES, VEGF, IL1b, IL- 6, IL-7, IL-8, IL-10, IL-13, G-CSF, MCP1, MIP1, ICAM1) by means of Bio-plex system (Bio-Rad). We have observed a significant correlation between oocyte maturation and the secretion of two immunosoppressive molecules: soluble HLA-G (sHLAG) and sICAM-1. sHLA-G has been observed in 14/73 (19%) mature COCs (300 - 800 pg/mL/24 hours) while no sHLA-G have been revealed in immature COCs (n = 39). sICAM-1 has higher levels in immature oocytes (6711.5 +/− 1502.4 pg/mL/24 hours) while it decreases during maturation (2987 +/− 103.7 pg/mL/24 hours). Interestingly these two molecules are inversely correlated (p = 0.0369; Spearman Correlation test) proposing a temporal control of molecule secretion to regulate the inflammatory microenvironment during oocyte maturation. These results support the concept that oocyte are protected from immunological events by the expression of sICAM and sHLA-G molecules which could act as immunosuppressive against the adhesion of cytotoxic lymphocytes and cell-mediated immunity respectively.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1398639
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