The T cell receptor (TCR) repertoires of cytotoxic responses to the immunodominant and subdominant HLA A11 -restricted epitopes in the Epstein- Barr virus (EBV) nuclear antigen-4 were investigated in four healthy virus carriers. The response to the subdomimant epitope (EBNA4 399-408, designated AVF) was highly restricted with conserved Vβ usage and identical length and amino acid motifs in the third complementarity-determining regions (CDR3), while a broad repertoire using different combinations of TCR-α/β V and J segments and CDR 3 regions was selected by the immunodominant epitope (EBNA4 416-424, designated IVT). Distinct patterns of interaction with the A11- peptide complex were revealed for each AVF- or IVT-specific TCR clonotype by alanine scanning mutagenesis analysis. Blocking of cytotoxic function by antibodies specific for the CD8 coreceptor indicated that, while AVF-specific TCRs are of high affinity, the oligoclonal response to the IVT epitope includes both low- and high affinity TCRs. Thus, comparison of the memory response to two epitopes derived from the same viral antigen and presented through the same MHC class I allele suggests that immunodominance may correlate with the capacity to maintain a broad TCR repertoire.

Epitope-dependent selection of highly restricted or diverse T cell receptor repertoires in response to persistent infection by Epstein-Barr virus

GAVIOLI, Riccardo;
1997

Abstract

The T cell receptor (TCR) repertoires of cytotoxic responses to the immunodominant and subdominant HLA A11 -restricted epitopes in the Epstein- Barr virus (EBV) nuclear antigen-4 were investigated in four healthy virus carriers. The response to the subdomimant epitope (EBNA4 399-408, designated AVF) was highly restricted with conserved Vβ usage and identical length and amino acid motifs in the third complementarity-determining regions (CDR3), while a broad repertoire using different combinations of TCR-α/β V and J segments and CDR 3 regions was selected by the immunodominant epitope (EBNA4 416-424, designated IVT). Distinct patterns of interaction with the A11- peptide complex were revealed for each AVF- or IVT-specific TCR clonotype by alanine scanning mutagenesis analysis. Blocking of cytotoxic function by antibodies specific for the CD8 coreceptor indicated that, while AVF-specific TCRs are of high affinity, the oligoclonal response to the IVT epitope includes both low- and high affinity TCRs. Thus, comparison of the memory response to two epitopes derived from the same viral antigen and presented through the same MHC class I allele suggests that immunodominance may correlate with the capacity to maintain a broad TCR repertoire.
1997
DE CAMPOS LIMA, P. O.; Levitsky, V.; Imreh, M. P.; Gavioli, Riccardo; Masucci, M. G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1204105
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