We purified an esterase from Bacillus acidocaldarius whose NH2-terminal sequence corresponds to an open reading frame (ORF) previously reported to show homology with the mammalian hormone sensitive lipase (HSL)-like subgroup of the esterase/lipase family. The enzyme displays esterase but not lipase activity. The enzyme we predicted the alpha/beta-hydrolase topological fold common to several esterases and lipases and identified Ser 155, Asp 252 and His 282 as the putative members of the catalytic triad.
Identification of an Esterase from Bacillus acidocaldarius with Sequence Similarity to a Hormone Sensitive Lipase Subfamily
ADINOLFI, Elena;
1997
Abstract
We purified an esterase from Bacillus acidocaldarius whose NH2-terminal sequence corresponds to an open reading frame (ORF) previously reported to show homology with the mammalian hormone sensitive lipase (HSL)-like subgroup of the esterase/lipase family. The enzyme displays esterase but not lipase activity. The enzyme we predicted the alpha/beta-hydrolase topological fold common to several esterases and lipases and identified Ser 155, Asp 252 and His 282 as the putative members of the catalytic triad.File in questo prodotto:
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