Prediction of the band profiles of the mixtures of the 1-indanol enantiomers from data acquired with the single racemic mixture

The adsorption isotherm data ofR- and S-1-indanol and oftheir racemic mixture on cellulose tribenzoate were measured by frontal analysis. These data were then fitted to the Langmuir, the Bilangmuir, the Toth, and the Langmuir-Freundlich isotherm models. The single component data fitted well to both the Bilangmuir and the Toth models. Combined with the lumped pore diffusion model (POR) of chromatography, these isotherms were used to calculate overloaded elution profiles of the pure enantiomers. The calculated and the experimental profiles agree excellently in all cases if the former are derived from the Bilangmuir model. The competitive experimental data also gave excellent agreement with the Bilangmuir model. The simultaneous fit ofall the data, for the single components and the racemic mixture, gave again superior agreement with the bilangmuir model. The overloaded elution profiles ofsamples ofthe racemic mixture calculated with the Bilangmuir isotherm model combined with the POR model ofchromatography gave results in very good agreement with the experimental band profiles oflarge samples ofthe racemic mixture. This confirms that in numerous cases the whole set ofcompetitive isotherms oftwo enantiomers can be derived from the experimental data obtained only with the racemic mixture.