Epidemiological and pharmacological studies have evidenced that colorectal cancer development could be reduced by consumption of vegetable containing glucosinolates. In this view, the effect of some glucosinolates and their isothiocyanate (ITC) derived products on in vitro cell growth was studied. We report the isolation and characterization of ITCs derived from glucosinolates by using HPLC, GC-MS and NMR techniques. The in vitro activity of ITCs on human erythroleukemic K562 cells have been investigated by using two alternative approaches, namely the in situ and pre-mix methods. No differences in antiproliferative activity were found comparing the effect of ITCs produced either by in situ or pre-mix method. In the experimental conditions used, the production of ITCs from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The ITCs inhibitory activity on K562 cells growth is particularly evident in the cases of ITCs derived from sinigrin, progoitrin, epi-progoitrin, glucotropaeolin and glucocheirolin. Finally, the antiproliferative activity of the ITCs obtained from glucoraphenin, taken as an example, was determined on other tumor cell lines with different origin and hystotype. Considering the antiproliferative activity found for ITCs, these compounds could be considered potentially responsible for the reduction of colorectal cancer associated to dietes rich in cruciferous vegetables. Further studies will be aimed to the possible application of glucosinolate derived products as chemopreventive cancer agents.
Myrosinase-generated isothiocyanate from glucosinolates: Isolation, characterization and in vitro antiproliferative studies
ESPOSITO, Elisabetta;MENEGATTI, Enea;CORTESI, Rita;NASTRUZZI, Claudio
1997
Abstract
Epidemiological and pharmacological studies have evidenced that colorectal cancer development could be reduced by consumption of vegetable containing glucosinolates. In this view, the effect of some glucosinolates and their isothiocyanate (ITC) derived products on in vitro cell growth was studied. We report the isolation and characterization of ITCs derived from glucosinolates by using HPLC, GC-MS and NMR techniques. The in vitro activity of ITCs on human erythroleukemic K562 cells have been investigated by using two alternative approaches, namely the in situ and pre-mix methods. No differences in antiproliferative activity were found comparing the effect of ITCs produced either by in situ or pre-mix method. In the experimental conditions used, the production of ITCs from glucosinolates is almost quantitative as confirmed by HPLC or GC-MS analysis. The ITCs inhibitory activity on K562 cells growth is particularly evident in the cases of ITCs derived from sinigrin, progoitrin, epi-progoitrin, glucotropaeolin and glucocheirolin. Finally, the antiproliferative activity of the ITCs obtained from glucoraphenin, taken as an example, was determined on other tumor cell lines with different origin and hystotype. Considering the antiproliferative activity found for ITCs, these compounds could be considered potentially responsible for the reduction of colorectal cancer associated to dietes rich in cruciferous vegetables. Further studies will be aimed to the possible application of glucosinolate derived products as chemopreventive cancer agents.I documenti in SFERA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
