This paper describes the production by the spray-drying technique of empty and trypsin-containing Eudragit RL microparticles. The effects of different air drying (heating) temperatures, namely 85, 95, 105 and 135°C, were evaluated. Both empty and trypsin-containing microparticles, characterized by optical and electron microscopy, exhibited the same morphological characteristics. Microparticles demonstrated particle recovery of between 38 and 67% and a size of between 5 and 11 μm. Microparticle recovery, morphological characteristics and mean diameters were not particularly infl uenced by heating. Indeed, in all cases, microparticles maintained a spherical shape, a slight increase in dimensions and no aggregation phenomena. However, an increase in air drying temperature caused a drastic reduction in terms of trypsin activity (assayed by UV determination) with respect to the free enzyme. Indeed, trypsin activity fell from more than 90 to 4.05% in the case of microspheres produced at 95 and 135°C, respectively. Nevertheless, using either suspended or solubilized polymer as the feeding material, the activity profi les of trypsin indicate that the enzyme maintained its activity almost quantitatively (93%) when particles are produced at 95°C. Taken together, these results suggest that spray-drying could represent an interesting technique for the production of microparticles intended for the administration of enzymes.

Spray-drying production of trypsin-containing microparticles

CORTESI, Rita;MENEGATTI, Enea;ESPOSITO, Elisabetta
2003

Abstract

This paper describes the production by the spray-drying technique of empty and trypsin-containing Eudragit RL microparticles. The effects of different air drying (heating) temperatures, namely 85, 95, 105 and 135°C, were evaluated. Both empty and trypsin-containing microparticles, characterized by optical and electron microscopy, exhibited the same morphological characteristics. Microparticles demonstrated particle recovery of between 38 and 67% and a size of between 5 and 11 μm. Microparticle recovery, morphological characteristics and mean diameters were not particularly infl uenced by heating. Indeed, in all cases, microparticles maintained a spherical shape, a slight increase in dimensions and no aggregation phenomena. However, an increase in air drying temperature caused a drastic reduction in terms of trypsin activity (assayed by UV determination) with respect to the free enzyme. Indeed, trypsin activity fell from more than 90 to 4.05% in the case of microspheres produced at 95 and 135°C, respectively. Nevertheless, using either suspended or solubilized polymer as the feeding material, the activity profi les of trypsin indicate that the enzyme maintained its activity almost quantitatively (93%) when particles are produced at 95°C. Taken together, these results suggest that spray-drying could represent an interesting technique for the production of microparticles intended for the administration of enzymes.
2003
Cortesi, Rita; Menegatti, Enea; Esposito, Elisabetta
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11392/1200242
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